Maryam Kakaei, Zahra Nazari, Latifeh Karimzadeh, M Nabiuni,
Volume 13, Issue 1 (5-2013)
Abstract
The in-vitro culture of immature ovarian follicles is used to examine the factors that regulate follicle development and may ultimately provide options for reproductive infertility. Normal follicular development of an oocyte that is capable of fertilization and embryonic development depends upon a complex sequence of cellular interactions within the follicle . Honey Bee Venom (HBV) contains some biological active components which has been demonstrated it,s promoting effect on ovulation up to 70 % in rats . The aim of this research is study of the effect of HBV on in-vitro maturation of different type of preantral follicles. In this research, unmature follicles were taken from 14 day-old mice and cultured in &alpha-minimal essential medium (&alpha-MEM) and divided to small, medium and large preantral follicles and treated with 1µg/ml BV. Then diameter of follicle was measured until 4th days. Finally, morphological appearance of mature oocytes was studied. Studies of follicle diameters and flowcytometry showed HBV led to an increase in follicle diameters (p<0.001). Amount of GV was 53 %(control group) and 31.4 (treatment group)that was significative (p<0.001). Developing up to meiosis I was 30% (control group) and 43.2% (treatment group) and developing up to meiosis II was 15% (control) and 22.3% (treatment group). In brief, we are confirmed that honey bee venom has led to significant changes in in-vitro maturation of preantral follicles and consequently, better performance for fertilization.
