Showing 2 results for کالوس
Volume 18, Issue 56 (9-2006)
Abstract
Methods of plants tissues and organs culture were used for micropagation, production of plants free from contamination especially viral contaminations preparation of plants for transforming, improvements and cloning. Culture of Canola has developed recently in Iran and is used for vegetable oil and protein. Our target in this study was micropropagation, induction of calli and plantlet regeneration by tissue culture. Explants of Pioneer cultivars from Canola were cultured in MS medium with different concentrations of PGRs (plant growth tegulators) such as IBA (Indol butyric acid), BA (benzyl adenin), 2,4-D (2,4 dichlorophenoxy acetic acid. Results of repeated experiments indicated that in MS medium with 2 mg/lit BA, and 1-2 mg/lit IBA plus 1 mg/lit BA induced white calli that become organogen when diameter reached to 5-6 mm. MS medium with 2 mg/lit BA and different concentrations of 2,4-D (2-10 mg/lit) induced yellow calli that were alike embryogen calli and either longitudinal or transversal sections proved embryogenic structures in primary steps in them. In MS medium with out of 2 mg/lit IBA induced brown calli that grew slow and died.
Volume 18, Issue 58 (8-2004)
Abstract
In this investigation, quantitative analysis of glycyrrhizic acid (GA) in roots and calluses from different populations of Glycyrrhiza glabra L. were performed by HPLC. For this purpose roots and seeds were collected from localities of Abadeh (Fars), Djupar (Kerman) and Veyssian (Lorestan). For callus production, root explants of ten days old seedlings were maintained on Murashige and Skoog medium with 2,4-D (0.5 mg.l-1), IAA (0.5 mg.l-1) and BAP (0.1 mg.l-1). After 42 days, the growing calluses were excised and used for GA analysis. High amounts of GA were found in roots but no significant differences were observed between roots from different populations contrary to calluses. Between calluses of different origin, the highest content of GA (still remaining very small compared with the roots one) was observed in callus of G.glabra L. var. glabra of Fars. The lowest level of GA was noticed in calluses of G.glabra L. var. glandulifera of Fars, which have shown the maximum of growth in vitro.
