S. Shokatyari, R. Heidari, Rashid Jamei, S. Hosseini Sarghein,
Volume 13, Issue 1 (5-2013)
Abstract
Alkaloids are a large group of molecules with biological, physiological and chemical activities. Plants of the genus (Podophyllaceae) are the known sources of alkaloids. In the present study the amount of total alkaloids of tubers of from two different regions of Iran, Marivan - Sanandaj and of from Sanandaj -Naghadeh was determined by spectrophotometry, on the reaction of alkaloid with bromocresol green (BCG). Moreover, the effect of some characteristicts of the soil habitat such as, soil pH, total nitrogen, potassium, altitude, texture soil, and nitrate content of tubers on the amount from total alkaloids was measured. The results of data analysis showed the significant differences between total alkaloid content of Marivan (27.12 ±1.18 mg/g D.W) with Sanandaj (17.42 ±0.77 mg/g D.W) and of Sanandaj (15.38 ±0.65 mg/g D.W) with Naghadeh (7.4 ±0.32 mg/g D.W). Also there was a significant difference between the species in different regions other than of Sanandaj (17.42 ±0.77 mg/g D.W) with of Sanandaj (15.38 ±0.65 mg/g D.W). Furthermore, the results show that nitrate content of was higher than that of. The soil texture of Sanandaj (L. armeniaca) was loam sand and soil of the rest regions was clay loam. All of soils were mildly alkaline. Results from the effect of environmental factors on the total alkaloid content showed that with increasing amount of total nitrogen and potassium of soil and nitrate content, total alkaloids will be reduced, whereas pH, texture soil and altitude had not significant influence on total alkaloid contents
Volume 18, Issue 44 (10-2009)
Abstract
Hyoscyamus is one of the medicinal plants, rich in tropane alkaloids. In this research the effects of nitrate and ammonium on production of secondry metabolites and other physiological parameters, were investigated. The results indicated that when nitrate and ammonium were both present in nutrient solution and ammonium concentration was lower than nitrate, dry and fresh weight of root and shoot increased. However, by increasing ammonium concentration, plant growth decreased. With increased concentra-tions of ammonium, root/shoot ratios were decreased. Nitrate reductase activity increased in both nitrate and ammonium fed plants. However, the higher ratio of ammonium to nitrate led to a decreased NRA. Alkaloid content (sum of hyoscyamine and scopolamine) increased in both nitrate and ammonium fed plants. However, the higher the ratio of ammonium to nitrate, the lower the alkaloid content.
