Farzaneh Najafi, R Khavarinejad, F Mohammadi,
Volume 12, Issue 3 (11-2012)
Abstract
In the present study, the effects of indole acetic acid (IAA) on certain physiological and biochemical parameters in Glycine max (L.) Merr under aluminum chloride (AlCl3)stress were studied. Seeds were sterilized and cultured in petri-dishes. Six days old seedlings were transferred to pots, and then they were irrigated with Hoagland solution in a growth chamber (with 16 h light period per 24 h, with day / night temperatures of 25/18 ºC respectively). Twenty days old plants were treated with different concentrations of AlCl3 (0, 50, 100, 200 and 300 µM) and IAA (0, 50 and 100 µM). Plants were harvested 15 days after treatment. The plants exhibited decline in the relative water content (RWC), protein, soluble sugars, the chlorophylls a and b, carotenoids contents and photosynthetic rate with increase of aluminum chloride concentration, but the respiration rate and CO2 compensation concentration were increased. With addition of IAA to culture solutions containing aluminum, the plants showed further decrease in the amount of soluble sugars, chlorophylls a and b, carotenoids and photosynthetic rate and further increase in other parameters. With increasing of aluminum concentration to culture solutions with and without IAA unsoluble sugars, proline and lipid peroxidation increased.
