E Hoveazi, M Nabiuni, K Parivar, M Masomi, J Agh,
Volume 15, Issue 1 (4-2015)
Abstract
Today, induced pluripotent cells (iPS) have been recognized as a new and good cell source for cell therapy. In this study, we examined whether human iPS cells, cultured on scaffolds, can differentiate into definitive endodermal cells, as precursor for hepatocytes, pancreatic and lung cells. Embryoid bodies (EBs) composed of iPS cells then EBs were seeded on electrospinning nanofiber scaffold (PCL). The cells were differentiated into definitive endoderm by using IDE1(induced definitive endoderm). Expression of definitive endoderm markers including Sox17, FoxA2 and Gsc were confirmed by Immunocytochemistry attaining and RT-PCR analysis. In the present study, cells morphology and cells viability were evaluated by using a scanning electron microscopy and MTT assay respectively.The results of this study demonstrated the positive effect of 3D cultures, by using suitable factors, on definitive endoderm differentiation.definitive endoderm by using IDE1(induced definitive endoderm). Expression of definitive endoderm markers including Sox17, FoxA2 and Gsc were confirmed by Immunocytochemistry attaining and RT-PCR analysis. In the present study, cells morphology and cells viability were evaluated by using a scanning electron microscopy and MTT assay respectively.The results of this study demonstrated the positive effect of 3D cultures, by using suitable factors, on definitive endoderm differentiation.
