Volume 8, Issue 1 (12-2009)
Abstract
This investigation was carried out to identify type and extent of mycorrhizal distribution in Tandooreh National Park and determine the effects of Ca and K concentration on mycorrhizal development in vitro on Zea maiz using natural soil as substrate. Soil and root samples were taken from top 30 cm soil using a 10cm diam. steel borer from 7 stations located at 11 50 to 2300m heights from sea level. At least 2 plots of 100m2 (10 ´10m) were sampled. Plant samples were also collected. Sections were prepared from root samples were longitudinally sectioned and stained with Lacto phenol cotton blue. Spores of mycorhizae were isolated usingcentrifugation60% in sucrose solution centrifugation at 9000 rpm for 2 min. Surface characteristics of spores were used in their identification. 53 species of plants were identified. Majority of plants (50] species had vesicular aruscular mycorrhizae (greater than 90%). Seven species of Glmous were present in the rhizosphrere of all stations. Seedlings of maiz were grown in the pots containing Tandooreh soil with or without addition of given amounts of Ca and K in a factorial statistical plan. After 8 weeks of cultivation in greenhouse conditions, the plants were removed and their roots were separated and stained using method of Hyman and Philips to detect type and extent of mycorrhization. Dry weight and nutritional elements were determined in roots and shoots. K and Ca added in certain concentrations to soil were effective on mycorrhizal development. Ca and K had synergistic ionic effects in lower concentrations and showed antagonistic ionic effects in high concentrations in mycorrhizal development. There was also a correlation between mycorrhizal development and absorbed amount of Ca and K in shoots.
Samaneh Rahmatzadeh, J Khara, Sk Kazemi Tabar,
Volume 14, Issue 3 (10-2014)
Abstract
Periwinkle (Catharanthusroseus L.) is one of the most important medicinal plants that commonly used for its anticancer alkaloids. In this investigation, the effects of different tryptophan concentrations (0, 150, 250 and 350 mg/l) under in vitro regeneration conditions have been studied. The regeneration of different explants on different media compositions in free tryptophan condition showed that the best media conditions obtained by nodal segments explants grown on MS medium containing (BAP, 0.5 mg/l) + (NAA, 1 mg/l). Also, we achieved the rooting medial composition as half MS medium supplemented with (IBA, 0.1 mg/l). After adding of tryptophan into this optimal medium, the maximum shooting and rooting percentages were obtained in the media containing 250 and 350 mg/l tryptophan, respectively. Furthermore, the measurement of shoot and root length, dry and fresh weight of shoot, fresh weight of root, chlorophyll a and carotenoid content and also, soluble sugars content of shoot and root, revealed the best medium in the presence of 350 mg/l tryptophan, while the maximum amount of root dry weight and chlorophyll b were obtained by 250/l mg tryptophan containing medium. . Finally, the analysis of total proteins content of shoot and root showed significant difference between all groups and the higher content of this for shoots and roots observed in 250 mg/l and 350 mg/l, respectively.
