Showing 4 results for Sugar
Volume 9, Issue 3 (10-2010)
Abstract
Biosurfactants are products of broad range of microorganisms. These compounds are surface active. Glycolipids, Phospholipids & Fatty acids, Lipopeptids and Lipoproteins, Polymeric biosurfactants and Special Biosurfactants are main types of biosurfactants. Rhamnolipid is a type of Glycolipids that is produced by Pseudomonas aeruginosa. In the present paper, P. aeruginosa MM1011 was obtained from Persian Type Culture Collection (Biotechnology Center, IROST). The aim of this investigation was optimization of mineral salts medium with sugar beet molasses to produce rhamnolipid considering specific parameters. such as: C/N, Temperature, pH, shaking rate, and Inoculation size. Since P. aeruginosa is Suc.(-), it was mutated to be able to use the sucrose as carbon source. The molasses treated chemically, then used by the bacterium. The results assayed by phenol-sulfuric acid method, and crude oil emulsification capability. Results of ANOVA. Depicted results showd that the optimum conditions without molasses is: temp.:33˚c, pH 7, C/N:18, shaking rate: 200 rpm and inoculom: 2% in 96 hours and the best medium is 3M which contained sugar beet molasses. Further investigation proved that the best production temperature is 33°c when pH is 6.8, shaking rate: 200 rpm, C/N: 16, inoculom: 2%. Using the above mentioned factors, the obtained results showed that the produced rhamnose was 0.22 g/L (rhamnolipid = 0.66 g /L) and crude oil emulcification was 55.5 %.
Volume 9, Issue 4 (10-2010)
Abstract
Nickel is one of the essential elements (micronutrients) for plant growth. In this study, the effects of different concentrations of nickel, (0, 40, 80, 160, 320, 640 and 1280&mu M) and some environmental factors such as pH and Ca2+ concentration on the growth of seedlings of four wheat cultivars (Sardari, Zarrin, Alamout and C-73-20) were investigated. Moreover, the impact of high concentration of Ni on root sugar secretion has been assessed using sulphuric-phenol method .The results indicated that Alamout was more resistant than the other cultivars regarding to nickel stress. On the other hand, root and shoot tissues showed different growth responses to Ni and radical growth was more sensitive than shoot growth. Decreased pH increased the impact effect of nickel on shoot and root growth. Our study showed that increasing Ca2+concentration decreases Ni toxicity. Finally, Ni inhibited the leakage of soluble sugars from root tissue.
Volume 18, Issue 56 (9-2006)
Abstract
Manganese (Mn) is a micronutrient that is essential for plant growth and develop-ment. Meanwhile, an excess amount of Mn is one of the most important growth limiting factors in acid soils. The present study was undertaken to identify the effects of excessive Mn on the physiological aspects of tea plant. To this end, 2-year old rooted cuttings of tea (Camellia sinensis L. assamica × sinensis) were grown in a modified Hoagland solution with pH=4.2 for 1 month with aeration in green house, with temperature of 27 ± 3 ˚C and photosynthetic photon flux of 101.5 µmol. m-2 s-1 (400-700 nm) at the plant level. After emergence of new, white roots with 5 to 10 cm in length, the plants were treated for 1 week with excessive Mn to a final concentration of 0.9 mM. After 1 week, the white roots were harvested and frozen in liquid N2 and kept at -80 ˚C until used for determination of PO activity, lignin and sugar as well as chlorophyll content. The activities of soluble peroxidase (SPO), covalently wall-bound peroxidase (CPO) and lignin content decreased by Mn treatment. On the other hand, the activities of ionically wall-bound peroxidase (IPO), sugar and chlorophyll contents increased by Mn treatment. These results suggest that excessive Mn may have beneficial effects on tea plant via lowering lignin content and increasing extensibility of the walls.
Volume 18, Issue 56 (9-2006)
Abstract
The mechanism of soybean(Glycine max L.cv.Gorgan 3) response to drought stress was evaluated. The plants were grown in pots under 23 ± 2°C temperature and 14000 lux light intensity and 14hr photoperiod. When first leaf appeard, three irrigation treatments occurred that consisted as one every day(control), once in three(mild stress) and in five days(sever stress).During 15 days the plants were irrigated an amount of 150 ml. The effect of irrigation treatment on the soluble sugar, total protein, proline, phenolic compound, chlorophyll content and nitrate reductase activity was studied.The results showed that in the root soluble sugar and proline content in mild and sever stress significantly increased while in the stem and the leaf these compound considarably increased under sever stress the total protein ,chlorophyll a,b and nitrate reductase activity in leaf especially decreased in sever stress. Also the decrease phenolic compound in leaf under this condition was significant
