The Effect Of High Intensity Aerobic Training On Mitochondrial DNA Copy Number In Visceral Adipose Tissue Of Ovariectomized Rats Fed With High-Fat Diet

talebi-garakani, elahe; kheradmand, shokoufeh; nasiri, khadijeh

doi:10.61186/jsmt.21.26.18

Volume 21, Issue 26 (12-2023) RSMT 2023, 21(26): 18-29 | Back to browse issues page

‎ 10.61186/jsmt.21.26.18

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talebi-garakani E, kheradmand S, nasiri K. The Effect Of High Intensity Aerobic Training On Mitochondrial DNA Copy Number In Visceral Adipose Tissue Of Ovariectomized Rats Fed With High-Fat Diet. RSMT 2023; 21 (26) :18-29
URL: http://jsmt.khu.ac.ir/article-1-587-en.html

The Effect Of High Intensity Aerobic Training On Mitochondrial DNA Copy Number In Visceral Adipose Tissue Of Ovariectomized Rats Fed With High-Fat Diet

Elahe Talebi-garakani

, Shokoufeh Kheradmand

, Khadijeh Nasiri

University of Mazandaran , UMZ.kheradmand@gmail.com

Abstract: (2527 Views)

The prevalence of obesity in postmenopausal women is higher compared to men. A decrease in estrogen with menopause leads to dysfunction in mitochondria. The purpose of this study is to investigate the effect of high intensity aerobic training(HIT) on the copy number of mitochondrial genome (mtDNAcn) and mitochondrial transcription factor A(Tfam) in visceral adipose tissue(VAT) in ovariectomized(OVX) rats fed with high-fat diet(HFD). 40 rats (8 weeks, 200±20 gr) were divided into 5 groups:1) normal diet (ND), 2) ND+OVX, 3) HFD, 4) HFD+OVX, and 5) HFD+OVX+HIT. HIT performed aerobic training (80-85% of maximum oxygen consumption) for 8 weeks/5 sessions/42 minutes.48 hours after the last training session, VAT samples were taken to evaluate mtDNAcn and Tfam gene expression by qRT-PCR method. ANOVA and LSD tests were used to compare the differences between groups. OVX and HFD didn’t lead to a significant change in Tfam gene expression, but caused a significant decrease in the mtDNAcn compared to the control group. HIT caused a significant increase in Tfam gene expression compared to the control group and Tfam gene expression and mtDNAcn compared to the HFD+OVX. Hence, HIT improves mitochondrial function through mtDNAcn and Tfam gene expression in VAT in OVX rats fed by HFD.

Keywords: Ovariectomized, Visceral Adipose Tissue, Intense Aerobic Exercise Training, mtDNAcn, Tfam

Full-Text [PDF 2102 kb] (449 Downloads)

Type of Study: Research | Subject: sport physiology
Received: 2023/06/6 | Accepted: 2023/08/23 | Published: 2023/12/31

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