fa تاثیر 8 هفته فعالیت ورزشی تدامی با شدت متوسط بر بیان پروتئین های IL-10 و HMGB1 و فعالیت آنزیم های SOD و GPx در هیپوکمپ رت های مبتلا به صرع فیزیولوژی ورزشی sport physiology پژوهشي Research <div style="text-align: justify;"><span style="font-size:14px;"><span style="font-family:IRANsans;"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="AR-SA"><span b="" lotus=""><strong>مقدمه و هدف:</strong> استرس اکسیداتیو و التهاب عصبی از مکانیسم‌های کلیدی در پاتوفیزیولوژی بیماری صرع هستند. فعالیت ورزشی در کنترل و بهبود علائم صرع نقش دارد. این مطالعه با هدف بررسی اثر تمرینات تداومی با شدت متوسط</span></span> <span lang="AR-SA"><span b="" lotus="">(</span></span><span dir="LTR"><span new="" roman="" times="">MICT</span></span><span lang="AR-SA"><span b="" lotus="">)</span></span> <span lang="AR-SA"><span b="" lotus="">بر فعالیت آنزیم&shy;های آنتی&shy;اکسیدانی سوپراکسیددیسموتاز (</span></span><span dir="LTR"><span new="" roman="" times="">SOD</span></span><span lang="AR-SA"><span b="" lotus="">)</span></span><span lang="FA"><span b="" lotus=""> وگلوتاتیون پراکسیداز (</span></span><span dir="LTR"><span new="" roman="" times="">GPx</span></span><span lang="FA"><span b="" lotus="">)</span></span><span lang="FA"><span b="" lotus="">، سطوح پروتئین&shy;های </span></span><span dir="LTR"><span new="" roman="" times="">HMGB1</span></span><span lang="FA"><span b="" lotus=""> و </span></span><span dir="LTR"><span new="" roman="" times="">IL-10</span></span><span lang="FA"><span b="" lotus=""> و شدت تشنج در هیپوکمپ رت&shy;های مبتلا به صرع انجام شد. </span></span></span></span></span><br> <span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><strong><span lang="FA"><span b="" lotus="">روش:</span></span></strong> <span lang="AR-SA"><span b="" lotus="">32 سر رت نر </span></span><span lang="FA"><span b="" lotus="">ویستار (سن: 6 تا 8 هفته و میانگین وزن: </span></span><span lang="FA"><span b="" lotus="">۲۲۶</span></span><span lang="AR-SA"><span arial="">٫</span></span><span lang="FA"><span b="" lotus="">۶۲۵</span></span><span lang="FA"><span calibri="">&plusmn;</span></span><span lang="FA"><span b="" lotus="">۱۴</span></span><span lang="AR-SA"><span arial="">٫</span></span><span lang="FA"><span b="" lotus="">۹۶۶</span></span><span lang="FA"><span b="" lotus="">) </span></span><span lang="AR-SA"><span b="" lotus="">به گروه‌های صرع، کنترل، </span></span><span lang="FA"><span b="" lotus="">شم</span></span><span lang="AR-SA"><span b="" lotus=""> و </span></span><span lang="FA"><span b="" lotus="">صرع + </span></span><span dir="LTR"><span new="" roman="" times="">MICT</span></span> <span lang="AR-SA"><span b="" lotus="">تقسیم شدند. پس از اتمام پروتکل هشت هفته&shy;ای تمرین، فعالیت </span></span><span dir="LTR"><span new="" roman="" times="">SOD</span></span><span lang="FA"><span b="" lotus=""> و </span></span><span dir="LTR"><span new="" roman="" times="">GPx</span></span> <span lang="AR-SA"><span b="" lotus="">با الایزا، سطوح</span></span> <span dir="LTR"><span new="" roman="" times="">HMGB1</span></span> <span lang="AR-SA"><span b="" lotus="">و </span></span><span dir="LTR"><span new="" roman="" times="">IL-10</span></span> <span lang="AR-SA"><span b="" lotus="">در بافت هیپوکمپ ب</span></span><span lang="FA"><span b="" lotus="">ا </span></span><span lang="AR-SA"><span b="" lotus="">وسترن&shy;بلات و شدت تشنج بر اساس مقیاس راسین اندازه گیری شد</span></span><span lang="FA"><span b="" lotus="">. داده&shy;ها از طریق آزمون تحلیل واریانس یک&shy;راهه و آزمون یومن&shy;ویتنی در نرم&shy;افزار </span></span><span dir="LTR"><span new="" roman="" times="">SPSS</span></span><span lang="FA"><span b="" lotus=""> تجزیه و تحلیل شدند.</span></span></span></span></span><br> <span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span lang="AR-SA"><span b="" lotus=""><strong>یافته‌&shy;ها:</strong> القای صرع منجر به کاهش فعالیت آنزیم‌های </span></span>&nbsp;<span dir="LTR"><span new="" roman="" times="">SOD</span></span><span lang="FA"><span b="" lotus=""> (0.02</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">)</span></span> <span lang="AR-SA"><span b="" lotus="">و </span></span><span dir="LTR"><span new="" roman="" times="">GPx</span></span> <span lang="FA"><span b="" lotus="">(0.001</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">)</span></span> <span lang="AR-SA"><span b="" lotus="">، افزایش پروتئین</span></span><span dir="LTR"><span new="" roman="" times="">HMGB1</span></span><span lang="FA"><span b="" lotus=""> (0.001</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">)</span></span> <span lang="AR-SA"><span b="" lotus="">، کاهش پروتئین</span></span> <span dir="LTR"><span new="" roman="" times="">IL-10</span></span><span lang="FA"><span b="" lotus="">(0.001</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">)</span></span>&nbsp; <span lang="AR-SA"><span b="" lotus="">و افزایش شدت تشنج شد. تمرین</span></span> <span dir="LTR"><span new="" roman="" times="">MICT</span></span> <span lang="AR-SA"><span b="" lotus="">&nbsp;موجب افزایش فعالیت آنزیم</span></span> <span dir="LTR"><span new="" roman="" times="">GPx</span></span> <span lang="FA"><span b="" lotus="">(0.048</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">) </span></span><span lang="AR-SA"><span b="" lotus="">و کاهش سطح </span></span><span dir="LTR"><span new="" roman="" times="">HMGB1</span></span> <span lang="FA"><span b="" lotus="">(0.001</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">) </span></span><span lang="AR-SA"><span b="" lotus="">در گروه تمرینی نسبت به گروه صرع شد. اگرچه فعالیت آنزیم </span></span><span dir="LTR"><span new="" roman="" times="">SOD</span></span> <span lang="FA"><span b="" lotus="">(0.953</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">) </span></span>&nbsp;<span lang="AR-SA"><span b="" lotus="">و سطح</span></span> <span dir="LTR"><span new="" roman="" times="">IL-10</span></span> <span lang="FA"><span b="" lotus="">(0.198</span></span><span dir="LTR"><span new="" roman="" times="">p < </span></span><span lang="FA"><span b="" lotus="">) </span></span><span lang="AR-SA"><span b="" lotus="">تغییر معنا‌داری نیافت، اما شدت تشنج در گروه تحت تمرین به طور قابل توجهی کاهش یافت.</span></span> <span lang="FA"><span style="font-family:"B Lotus""></span></span></span></span></span><br> <span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><strong><span lang="FA"><span b="" lotus="">نتیجه‌گیری:</span></span> </strong><span lang="AR-SA"><span b="" lotus="">به نظر می‌رسد تمرینات تداومی با شدت متوسط با تقویت سیستم آنتی‌اکسیدانی</span></span> <span lang="AR-SA"><span b="" lotus="">و کاهش</span></span> <span lang="AR-SA"><span b="" lotus="">التهاب عصبی، اثرات محافظتی خود را اعمال کرده و باعث کاهش شدت تشنج در </span></span><span lang="FA"><span b="" lotus="">رت&shy;های مبتلا به صرع</span></span><span lang="AR-SA"><span b="" lotus=""> می&shy;شود.</span></span></span></span></span></span></span></div> <span style="font-size:12pt"><span style="line-height:normal"><span style="font-family:Aptos,sans-serif"><b><span style="font-size:13.0pt"><span arial="" style="font-family:">Abstract</span></span></b></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="font-family:Aptos,sans-serif"><b><span style="font-size:13.0pt"><span arial="" style="font-family:">Introduction: </span></span></b><span style="font-size:13.0pt"><span arial="" style="font-family:">Oxidative stress and neuroinflammation are key mechanisms in the pathophysiology of epilepsy. Physical activity plays a role in controlling and improving epileptic symptoms. This study aimed to investigate the effects of moderate-intensity continuous training (MICT) on the activity of antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), the levels of HMGB1 and IL-10 proteins, and seizure severity in the hippocampus of epileptic rats.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="font-family:Aptos,sans-serif"><b><span style="font-size:13.0pt"><span arial="" style="font-family:">Methods</span></span></b><span style="font-size:13.0pt"><span arial="" style="font-family:">: Thirty-two male Wistar rats (6&ndash;8 weeks old; mean body weight: 226.625 &plusmn; 14.966 g) were randomly assigned to four groups: epilepsy, control, sham, and epilepsy + MICT. After completion of the 8-week training protocol, SOD and GPx activities were measured by ELISA, hippocampal HMGB1 and IL-10 levels were assessed by Western blotting, and seizure severity was evaluated using the Racine scale. Data were analyzed using one-way analysis of variance (ANOVA) and the Mann&ndash;Whitney U test in SPSS.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="font-family:Aptos,sans-serif"><b><span style="font-size:13.0pt"><span arial="" style="font-family:">Results: </span></span></b><span style="font-size:13.0pt"><span arial="" style="font-family:">Epilepsy induction led to decreased SOD (p < 0.02) and GPx (p < 0.001) activities, increased HMGB1 levels (p < 0.001), decreased IL-10 levels (p < 0.001), and increased seizure severity. MICT increased GPx activity (p < 0.048) and reduced HMGB1 levels (p < 0.001) in the training group compared with the epilepsy group. Although SOD activity (p = 0.953) and IL-10 levels (p = 0.198) did not change significantly, seizure severity was significantly reduced in the trained group.</span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="font-family:Aptos,sans-serif"><b><span style="font-size:13.0pt"><span arial="" style="font-family:">Conclusion: </span></span></b><span style="font-size:13.0pt"><span arial="" style="font-family:">Moderate-intensity continuous training appears to exert protective effects and reduce seizure severity in epileptic rats by strengthening the antioxidant defense system and attenuating neuroinflammation.</span></span></span></span></span> صرع, فعالیت ورزشی تداومی با شدت متوسط, التهاب, آنتی­ اکسیدان, تشنج Epilepsy, Moderate-Intensity Continuous Training, Inflammation, Antioxidant, Seizure 0 0 http://jsmt.khu.ac.ir/browse.php?a_code=A-10-2560-1&slc_lang=fa&sid=1 Younes Sarkabood یونس سرکبود Younessarkabood@ut.ac.ir 10031947532846006297 10031947532846006297 No University of Tehran دانشگاه تهران Mohamadreza Kordi محمدرضا کردی mrkordi@ut.ac.ir 10031947532846006298 10031947532846006298 Yes University of Tehran دانشگاه تهران Siroos Choobineh سیروس چوبینه choobineh@ut.ac.ir 10031947532846006299 10031947532846006299 No University of Tehran دانشگاه تهران Fatemeh Nasery fatemehnasery283@gmail.com 10031947532846006300 10031947532846006300 No University of Tehran دانشگاه تهران