Training exercises are known to affect depressed girls condition, but the effect of regular exercise in exhilarating environments, in particularly on markers related to epinephrine metabolism in depressed girls are not well known. The purpose of the present study was to determine effect of the 6 weeks of intermittent walking in water on urine MHPG sulphate and Hamilton scale in the depressed girls. Twenty-four of girls' students, who suffering from moderate depression, randomly divided into exercise and control groups. The subjects in the training group performed the progressive walking exercise of 60-70% of maximal heart rate, 3 times a week and for 6 weeks. The Hamilton scale and urine samples were measured in before and after the mentioned exercise protocol. The urine MHPG sulphate was measured by HPLC-flurometric detection method. The data were analyzed by t- student and the Pearson correlation coefficient tests at p≤0/05 levels. The results showed the 6 weeks of exercise resulted in significant increase in the urine MHPG sulphate and VO2max and significant decrease in Hamilton scale compared to the control group. Furthermore, there was a significantly positive correlation between the urine MHPG sulphate and VO2max (r= 0/65). However, there was a significant reversely correlation (r= - 0/52) between the urine MHPG sulphate and Hamilton scale in prediction of depression. These results emphasize the role of regular physical exercise, particularly in exhilarating environments in the promotion of depression state. Also, in some conditions can be used Hamilton scale instead of the urine MHPG sulphate detects.
