Dr Sajad Karami, Dr Hamid Rajabi, Dr Majid Kashef, Dr Mohammad Ali Gharaat,
Volume 0, Issue 0 (11-2019)
Abstract
Introduction and purpose: The present study was conducted with the aim of investigating the effect of acute glutamine supplementation along with intermittent exercise on Hsp70 and blood lactate response of club football players. Methodology: 32 players of the Tehran Railway team were selected and randomly divided into four groups: control, supplement, supplement-sports activity, and sports-activity. Subjects participated in daily exercises before conducting the study, and only two complementary groups - sports activity and sports activity - performed the periodic protocol. Blood samples were taken at baseline, pre-test, post-test and 90 minutes after the test. The supplement and placebo were consumed in an amount of 0.5 g/kgBW and a volume of 5 ml/kgBW one hour before the periodic protocol. The interval protocol included 3 stages of 20-minute running with an intensity of 80% of the maximum heart rate and a 5-minute rest of walking between the stages. HSP72 and blood lactate levels were measured using ELISA and enzymatic methods, respectively. The data were analyzed using the analysis of variance test with repeated measures and the between-group factor at the P≤0.05 level. Findings: Glutamine supplement alone and together with exercise stimulate the response of HSP72 and the combination of supplement and exercise leads to a greater response of HSP72 and lactate changes did not play a role in this response. Discussion and conclusion: It seem that taking glutamine supplements in the long term creates more adaptations, which of course should be studied.
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Volume 12, Issue 7 (4-2014)
Abstract
The aim of the present study was to investigate the effect of glucose and glutamine supplementation on serum HSP72, in non-athlete men during four weeks exhaustive endurance - intermittent training that causes glycogen depletion. For this purpose 20 non-athlete healthy men were selected and randomly divided into four groups including: glucose supplementation with glycogen depletion training group (n = 5, supplement 1), glutamine supplementation with glycogen depletion training group (n = 5, supplement 2), glycogen depletion training group (n = 5, placebo) and a group without any treatment (n = 5, control). The blood samples were collected at the onset of training protocol period and 48 hours after final training session from anticubital venous. Serum HSP72 concentration were determined by ELISA technique. The main and interaction effects of variables were determined using TWO WAY ANOVA compeleted with Tukey post-hoc test. The significant level was chosen as α = 0.05.The results showed the significant effect of training variable for serum HSP72 concentration, [F(1,17)= 6.362 P<0.05] . The effect of supplementation variable was significant for serum HSP72 concentration, [F(1,17)= 15.362 P<0.01] . Post-hoc test showed the significant difference between placebo with supplement 1 group (p<0.05) and placebo with supplement 2 group (p<0.01). The interaction effect between two variables was not significant. In summery, the results suggested that in glycogen depletion training condition, glucose or glutamine supplementation have significant effect on serum HSP72 concentration.
