Zahra Khadivi Burojeny, Hamid Rajabi, Mohammad Marandi, Shaghayegh Haghjoo, Alireza Khadivi Burojeny, Ebrahim Noorian,
Volume 16, Issue 15 (9-2018)
Abstract
The purpose of present study was to determine the effect of 8 weeks of resistance training on serumic myostatin, and Fibroblast Growth Factor-2 (FGF-2) level in male Wistar rats. 20 adult male wistar rats(150-250gr) were randomly divided into 2 groups [control group; n=10 (C), resistance training; n=10 (R)]. Resistance training was conducted for 8 weeks (5 session/week) on a special 1 meter height ladder (divided by 26 stairs) with loading of 30٪ of body weight (suspended from the tail) in the first week and increased to 200٪ of body weight in the last week. Training includes 3 sets of 4 reps with 3 minutes rest between sets. At the end of last week blood samples were taken from the rats and myostatin, TGF-β1 and FGF-2 was measured in three groups with the ELISA kit. At the end of the study ،the plasma levels of the myostatin decreased [71/82±19/62 mg/dl (R) ver. 105/86±17/49 mg/dl(C). (p≤0/001)], but the level of FGF-2 increased significantly in resistance training group [102/462±11/135mg/dl (R) ver. 86/96±12/606 mg/dl (C) (p=0/048)]. In contrast, the serum level of TGF-β was not statistically different between the two groups [153/48±54/09 mg/dl (R).160/62±32/85 mg/dl (C). (p=0/725)]. This study shows that 8 weeks of resistance training reduces serum levels myostatin and increased serum levels of FGF-2. These two factors cause activation or non-activation of satellite cells (Muscle stem cells that control muscle hypertrophy). However the nerve - muscle agents in the first 8 weeks of resistance training have the maximum participation on strength increases, but in this research we see affect the signals of satellite cells in the first 8 weeks of resistance training can change significantly. Also, although serum levels of TGF-β1 in the exercise group was significantly different from the control group, but the overall value of the exercise group had slightly reduced.
Mohsen Soltani, Ali Barzegari, Saeed Naghibi, Mohammad Hassaan Dashty Khavidaki,
Volume 21, Issue 26 (12-2023)
Abstract
This study aimed to investigate the effect of quercetin supplementation and training on myostatin and follistatin levels in the heart tissue of diabetic rats. Methods: For this purpose, 50 10-week-old male Wistar rats with an average weight of 237±33 grams were randomly divided into five groups of 10 including: healthy control, diabetes, diabetes+supplement, diabetes+exercise, and diabetes+supplement+exercise. . The training protocol includes five sessions per week for eight weeks on a treadmill without a special slope. The rodents ran for 60 minutes at a speed of 25 meters per minute in each session.and the supplement groups received 50 mg/kg quercetin daily by gavage. The results showed that the difference in myostatin levels among the groups is significant (P=0.001). There was a significant decrease in myostatin levels between exercise+supplement+diabetes group with diabetes (P=0.002). There was also a significant difference between the supplement+diabetes and exercise+diabetes groups (P=0.009). Also, the results showed that the difference in follistatin levels among the groups is significant (P=0.001). The results showed that follistatin levels in the heart tissue increased significantly between the groups of diabetes, exercise+diabetes, supplement+diabetes and exercise+supplements+diabetes with the control group (respectively: P=0.005; P=0.001; P=0.001; P = 0.001).
It seems that moderate intensity aerobic exercise and quercetin supplementation have a positive and beneficial effect on the levels of myostatin and folstatin.
