One of the ways to optimize sports activities is the use of nanotechnology. Silver and zinc oxide nanoparticles have valuable effects in improving sports performance of athletes. The purpose of this research was to investigate the effect of clothes containing silver and zinc oxide nanoparticles on the physiological responses and sports performance of female athletes. The statistical population of the research was women active in public sports in Maku city. The sample size was selected by random method and 10 female athletes (with an average age of 25.9, an average weight of 60 kg and a body mass index of 25.49) were selected. The effect of the treatments (sports activity with and without nano clothes) on endurance, moisture absorption, bacterial removal, systolic and diastolic blood pressure and heart rate of the athletes were investigated. The results showed that the use of clothes containing silver and zinc oxide nanoparticles increases their endurance. These clothes lead to an increase in bacteria removal in them. The clothes containing these nanoparticles were effective in reducing their blood pressure. It also improved moisture absorption in female athletes. In general, clothes containing silver nanoparticles and zinc oxide nanoparticles have an effect on reducing the physiological responses and sports performance of female athletes.