Saponins are secondary metabolites that are found in many plants and some animals. These compounds are high molecular weight glycosides, consisting of a sugar moiety linked to a triterpene or steroid aglycone. Many saponins have detergency properties and give stable foam in water. The genus Silene L. with more than 700 species is one of the largest genera of the family Caryophyllaceae mainly distributed in northern hemisphere. Saponins are one of the important secondary metabolites in the members of the genus. Quantitative and qualitative study of the saponins in the aerial parts and roots of three Silene species, namely S. ginodioica Ghaz. subsp. Penducularis (Fenzl ex Boiss.) Melzh., S. spergulifolia (Willd.) M. Bieb. and S. swertiifolia Boiss. were carried out by spectrophotometry and TLC methods. In this study, 5 fractions were obtained from each plant part and in both parts of the three species, fraction 1 contains the highest amounts of saponins. On the other hands, root extracts have high amounts of saponins. In addition, thin layer chromatography (TLC) of the extracts revealed some saponins spots with different R_f.