Effect of adding cysteine to Low Density Lipoprotein-based extender on goat sperm quality after freezing

Ghorbanzadeh, Javad; Zeinoaldini, Saeed; Zhandi, Mahdi; Hussaini, Sayyed Mohammad Hadi

Back to the articles list | Back to browse issues page

Effect of adding cysteine to Low Density Lipoprotein-based extender on goat sperm quality after freezing

Javad Ghorbanzadeh

, Saeed Zeinoaldini ^*

, Mahdi Zhandi

, Sayyed Mohammad Hadi Hussaini

University of Tehran , zeinoaldini@ut.ac.ir

Abstract: (23 Views)

This study aimed to investigate the effect of adding cysteine to an Low Density Lipoprotein (LDL)-based extender on some quality parameters of goat sperm after freezing. This study was conducted in a completely randomized design with four treatments, including: egg yolk-based extender without cysteine (EY), LDL-based extender containing zero (LDL-C0), five (LDL-C5), and 10 (LDL-C10) mM cysteine, and six replicates. Semen samples after dilution with the aforementioned extenders, were frozen. After thawing, total and progressive motility, sperm plasma membrane integrity, functionality, and sperm morphology were evaluated. Total motility was higher in LDL-C5 compared to EY and LDL-C10 (P <0.05). Progressive motility in all treatments using LDL-based extender was significantly higher than EY. Other motility parameters were higher in LDL-C5 compared to EY (P <0.05). However, no significant differences were observed between the LDL-C0, LDL-C5, and LDL-C10, except for the VSL, which was significantly lower in LDL-C0 compared to LDL-C5 (P <0.05). Sperm plasma membrane integrity, functionality, and sperm morphology were higher in all treatments using LDL-based extender compared to EY (P <0.05), with the highest values observed in LDL-C5. Overall, the results showed that adding five mM cysteine to the LDL-based extender can improve some quality parameters of goat sperm.

Keywords: Antioxidants, Freesing, Goat, Quality, Sperm