Rezvan Ghannad, Forough Akbari , Maryam Madadkar Haghjou,
Volume 3, Issue 4 (12-2016)
Abstract
The effects of some pretreatments including blue-green and green algae and minerals on the induction of me-tabolic and biochemical process of germination were studied in Dracocephalum kotschyi Boiss. seeds. The seeds were pretreated with green algae Spirulina platensis, Chlorella vulgaris and Dunaliella bardawil or D. IR-1 (Isolated from Gave-Khooni-salt marsh) and modified Johnson nutrient medium. Dunaliella species belong to salt marshs and waters, hence their medium was prepared by means of 0.6 % NaCl. Spirulina's nutrient medium (NM) included 0.1% NaCl. The activities of α-amylase, ß-amylase, dehydrogenase, content of total sugar, reducing sugars and total protein (all we-re measured after priming) increased in comarsion with control. Treatment without NaCl and the treatment with 0.1 % NaCl caused the highest amount of the parameters mentioned. In the presence of salt (often at 0.6 % NaCl), algae could not improve and increase the parameters. In contrast, in the absence of salt, the positive effects of algae increased. Ge-rmination indices and length of shoots and roots which were measured after sowing the seeds into Petri-dishes, showed some positive effects regarding the pretreatments. Spirulina cells filteration with or without NM (including 0.1% NaCl) caused the highest activities in α-amylase and ß-amylase and the highest amount of total sugar and FGP. Chlorella filt-eration led to the increment of dehydrogenase activity and also the root's lenght. Dunaliella cells filteration in NM (without salt) increased α-amylase and ß-amylase activities and the reducing sugars.
Ms Afsaneh Teymouri, Dr Atefe Amirahmadi, Dr Reza Naderi, Dr Fatemeh Salimi, Dr Reza Sheikhakbari-Mehr,
Volume 12, Issue 1 (3-2025)
Abstract
The identification and introduction of medicinal plants hold significant importance in modern life. The genus Pycnocycla, belonging to the Apiaceae family, includes eight endemic species in Iran. P. spinosa has attracted attention due to its medicinal properties. This study aims to investigate the effect of three habitats (the Damghan, Semnan, and Kerman city) on the antimicrobial properties of extracts obtained from Pycnocycla spinosa using different solvents. To examine the antimicrobial properties, methanolic, ethyl acetate, and hexane extracts at a concentration of 2 mg/ml were tested against two pathogenic Gram-negative bacteria, Yersinia enterocolitica and Salmonella enterica, using the agar disk diffusion method. The results indicated that the extracts had varying effects on the bacteria. Additionally, the different geographical regions of Iran, due to their distinct climatic conditions, exhibited varying effects on the bacteria. Extracts from Kerman, especially those using methanol and ethyl acetate as solvents, showed the highest antimicrobial activity against Salmonella enterica. Therefore, the type of solvent and habitat can significantly influence the antimicrobial properties of plants.