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Behnam Hamzeh’ee, Mostafa Koshnevis, Parvaneh Ashouri, Vailolah Mozaffarian, Hooman Ravanbakhsh,
Volume 7, Issue 1 (4-2020)
Abstract

Fire affects vegetation and changes plant succession. In this paper, the vegetation of Sirachal Research Station, which burned in the summer of 2014, was studied and compared with the unburnt areas, based on biodiversity indices. The research was implemented as a factorial experiment in a completely randomized design. First, on the basis of physiognomy, the area was divided into three parts: shrubland, shrubland-rangeland, and rangeland. Each part was, then, divided into two sections, including a burnt area and an adjacent unburnt area (control area). In each area (burnt and unburnt), three sampling units were randomly assigned using PNP method, and vegetation measurements were subsequently performed. Based on data analysis, a total of 141 taxa were identified, belonging to 28 families and 95 genera, including one species of Gymnosperm, 19 monocotyledons and 121 dicotyledons in two burnt and unburnt areas of Sirachal Station. Based on the statistical analysis, using Past3 and SPSS softwares, there was no significant difference in the number of taxa between the vegetation of the burnt and unburnt areas. Of the indicators analyzed in Past3, the number of individual, the index of Menhinick's richness and the Shannon diversity index were significantly different in the treatments sampled from the burnt and unburnt areas. According to the Duncan test average, the number of individuals in the unburnt area, with a canopy cover below 25%, was greater than that in the burnt area, with the same canopy cover, however, no significant difference was observed between the individuals in the burnt area and unburnt area, with the same canopy cover of more than 25%. Also, the richness of the burnt area with a canopy of less than 5% is significantly higher than that in the unburnt area with the same canopy cover.
 
 
 
Parissa Jonoubi, Majid Ghorbani Nohooji, Halimeh Hassanpour, Atefeh Ashourisheikhi,
Volume 12, Issue 2 (9-2025)
Abstract

Ferula gummosa Boiss. as a valuable pharmaceutical and industrial plant grows in Iran. In order to minimize seed dormancy period and micropropagation, callus induction and embryogenesis were evaluated. The plantlets of the seeds were separated and cultured in the 1/2 MS medium. After 14 days, root, hypocotyl, cotyledon and leaf explants were separated. Then they were transferred to the basal MS medium containing different concentrations of growth regulators. Different developmental stages of somatic embryos were evaluated. Seedlings 2-3 days after locating in 1/2 MS medium germinated and whole plantlets were obtained after 12 days. In the callus induction phase growth regulator composition 1.5 mgl-1 of NAA and 0.5 mgl-1 of BA with root explants had proper results. In the somatic embryogenesis phase MS medium containing 0.5 mgl-1 of 2,4-D accompanied by 1 mgl-1 of BA lead to desirable results. Via taken sections from the embryos, different developmental stages of somatic embryos including pre-embryo, globular, heart shaped, torpedo and cotyledonary embryo were observed. In vitro culture of embryo to accelerate germination and elimination of long dormancy period and using this optimized method are strongly suggested for micropropagation of this plant so this valuable endemic plant may survive from extinction, too.
 

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