Ayuob Mazaraie, Seyed Mohsen Mousavi-Nik, Leila Fahmideh,
Volume 4, Issue 4 (3-2018)
Abstract
Phenolic compounds, especially those with plant origin, constitute an essential part of the human diet due to their antioxidant properties. Antioxidants protect biological systems (nucleic acids, proteins, lipids, lipoproteins) against free radicals and reactive oxygen species. Some medicinal plants contain so high amounts of antioxidants that their use can be considerably effective for human health. The aim of this study was to evaluate the effect of flooding extraction method on phenolic compounds, flavonoids and the level of free radical scavenging properties of medicinal plants including Thyme, Hamadani Yarrow, Shirazi mountain thyme, Yarrow, Chamomile, Saffron corm hyssop Sagebrush, wormwood, Artemisia and Ziziphora clinopodioiedes, in four extraction solvents including water, methanol, acetone and ethanol. The results showed that methanol and water solvents had the highest and the lowest total phenolic, flavonoid and antioxidant activity, respectively. The results also showed that methanol extraction of Shirazi thyme and water extraction of Wormwood plant had the highest and the lowest phenol and flavonoids contents, respectively. The results of trapping the free radicals of DPPH indicated that the methanol extract of Shirazi thyme and aqueous extract of wormwood had the highest and the lowest inhibition activities, respectively. Therefore, the selection of solvent type can affect the rate of the extraction of phenolic compounds, flavonoids and antioxidant activity.
Zahra Noormohammadi, Mahnoosh Mohammadzadeh-Shahir, Donya Fahmi, Seyed Mohammad Atyabi, Farah Farahani,
Volume 6, Issue 3 (10-2019)
Abstract
Catharanthus roseus is widely cultivated around the world. Cold atmosphere plasma (CAP) has been used to improve crop plants seed germination, and plant growth. In the present study, three different times of CAP (40, 50 and 60s) were used for the treatment of C. roseus seeds, and the changes in morphological traits, antioxidant enzymes and the genetic content of the treated plants were examined. Cold plasma (50s) markedly raised the seed germination, length of the leaves and the stem length of the plants in comparison with those in the control group. The catalase and peroxidase enzyme activities had the highest value in 60 and 40 s CAP treated plants, respectively. The sequence related amplified polymorphism (SRAP) markers showed the highest degree of genetic diversity in 50s cold plasma treated plants (Ne = 1.388, I = 0.316, He = 0.217, uHe = 0.237 and P % = 50.08). Based on Neighbor Joining, principle coordination analysis (PCoA) and analysis of molecular variance (AMOVA) test, four significantly distant groups were formed. The 40s and 50s cold plasma treated plants stand far from the control plants due to genetic difference. The results indicate that cold atmosphere plasma could be used as an economic and environmentally safe tool in increasing C. roseus growth characteristics in addition to inducing genetic variations.
