Showing 2 results for Gholinezhad
Fereshteh Heidargholinezhad, Yousef Hamidoghli, Valliollah Ghasemiomran, Pouria Biparva,
Volume 9, Issue 4 (12-2022)
Abstract
Centella asiatica, is well known to be a valuable medicinal plant for producing valuable compounds such as asiaticoside, asiatic acid, madcasoside and madcasic acid. The plant is believed to improve memory, lower blood pressure, be a strong antioxidant and anticancer. Therefore, it is important to optimize tissue culture methods in order to facilitate the extraction of medicinal compounds, gene transfer as well as improvement of medicinal properties of the plant. Calli prepared from various medicinal plants can be used to increase the amount of medicinal compounds in the cell suspension culture and gene transfer. The aim of this study was to investigate the effect of different concentrations of two hormones, BAP and NAA, on leaf explant for callus initiation, as one of the important sources of secondary metabolites production. For this purpose, leaf explants were treated with 6 different concentrations of BAP (0, 0.5, 1, 1.5, 2.5, 3.5 mg/L) and 5 different concentrations of NAA (0, 0.25, 0.5, 1, 2 mg/L). The results of this study showed that the best callus was obtained by the combination 1.5 mg/L of BAP and 0.5 mg/L of NAA, resulting in the leaf explants with callus induction of 100%, fresh weight of 1.457 gr and callus diameter of 1.459 cm. The results showed that two hormones of BAP and NAA have synergistic effects on the increase of the quality and quantity of the produced calli.
Naser Samsami, Jalal Jalilian, Esmaeil Gholinezhad, Raheleh Tahmasebi,
Volume 12, Issue 3 (9-2025)
Abstract
This study was conducted in a randomized complete block design with three replications with aimed to investigate the effects of different levels of salinity stress on root traits, allometric coefficient, and grain yield of Galega in three cultivation conditions (greenhouse, outdoor pots, and field) during 2024 cropping year. The results of variance analysis in greenhouse conditions showed that salinity stress had a significant effect on root diameter, root dry weight, and leaf dry weight. Specifically, salinity stress of 10 and 5 dS/m caused a 49 and 16 percent reduction in root diameter, 27 and 8 percent in root dry weight, and 49 and 10 percent in leaf dry weight, respectively. In outdoor pots, salinity stress had a significant effect on stem length, root volume, root diameter, root dry weight, stem dry weight and grain yield, and significant reductions were observed in these traits, reaching a maximum of 69% in stem dry weight. In field conditions, similar results were obtained, and salinity stress of 10 and 5 dS/m, respectively, caused a 78 and 67% reduction in grain yield and significant changes in other traits. Therefore, field cultivation conditions and lower salinity stress levels (below 5 dS/m) were superior for planting Galega.
