Showing 6 results for Sadeghi
Fateme Sadeghipour, Navaz Kharazian, Saeid Afsharzadeh,
Volume 5, Issue 3 (12-2018)
Abstract
The Palang Galoun protected region, comprising 34935 hectares, is located 75 km northwest of Najaf Abad, and 102 km northwest of Isfahan city. The aim of this research was to assess the floristic spectrum, life form varieties, chorological analysis, determination of protected status and identification of medicinal, threatened species criteria and poisonous plants of this natural heritage. Plant samples were collected during different vegetative seasons at several stages. Life forms of samples were determined and chorological analysis was performed. On the basis of the obtained results, a total of 166 species belonging to 126 genera and 39 families were identified. Six families, 23 genera and 26 species belonged to monocotyledons, whereas 33 families, 103 genera and 140 species belonged to dicotyledons. In terms of chorological analysis, 58% of plant species were distributed in the Irano-Turanian region. It is noteworthy that 44 endemic species, 97 medicinal species, 48 pasture species and 23 poisonous species were present in the studied area. Life forms were found to comprise 54% hemicryptophytes, 24% therophytes, 10% geophytes, 7% chamaephytes and 5% phanerophytes. Based on IUCN red list categories and criteria, there were 22 species in the lower risk category and seven species categorized as vulnerable case.
Mohsen Fatemi, Nasrin Mollania, Madjid Momeni-Moghaddam, Fatemeh Sadeghifar,
Volume 6, Issue 1 (5-2019)
Abstract
New properties of nano-materials have made nanotechnology the leading part of biology and medical sciences. Due to their various biomedical properties, iron-based magnetic nanoparticles (MNPs) have been highly considered by biological researchers. Nowadays, increasing resistance to antibiotics is a major problem in treating clinical infections. Finding new antibacterial agents is therefore essential for the treatment of resistant strains. In this study, the iron oxide MNPs were produced using culture-medium supernatant of a newly isolated bacterium to investigate the inhibitory effects of the NPs on strains with a major role in clinical infections. Biosynthesis of iron oxide MNPs were detected by UV-Vis spectroscopy and the average size of particles was estimated by dynamic light scattering technique. The anti-bacterial activity of these NPs against E. coli and S. aureus was investigated using methods for the calculation of bacterial sensitivity coefficient. In the presence of NPs, the highest sensitivity coefficient value was observed for E. coli in 1xMIC concentration. On the other hand, S. aureus showed the lowest value. The death rate of the two strains in contact with NPs followed the first order kinetic equation and the survival rate decreased with the increase of exposure time. The results of this study as well as the high functionality of iron oxide MNPs, make its application desirable in the prevention and treatment of clinical infections.
Solmaz Khosravi, Parisa Koobaz, Davood Naderi, Narges Mojtahedi, Akram Sadeghi,
Volume 6, Issue 1 (5-2019)
Abstract
The availability of iron for roots has been demonstrated as a critical factor in plant production. The addition of synthetic iron chelates to soil is a common practice in agriculture, which is not economically beneficial. Besides, chemical iron fertilizers cause many problems such as food contamination and environmental pollution. Development of natural Ferrioxamine B as an efficient and safe iron source may be the best strategy to overcome plant iron deficiency and prevention of synthetic agent pollution. The present study investigates the ability of a hydroxamate type siderophores (Ferrioxamine B) as a substitute Fe source during tissue culture of chickpea plants. For this purpose, embryo axes from chickpea seeds were surface sterilized and cultured in 1/2MS and MS culture media including 3% sucrose and 0.8% agar with Ferrioxamine B or Fe–EDTA. The root and shoot length, shoot and root dry weight, total fresh and dry weight, as well as chlorophylls a and b were analysed. Results indicated that Ferrioxamine B did not increase chlorophylls a and b in comparison with Fe-EDTA. However, rooting (22%), total dry weight (38%) and root (75%) and shoot (22%) dry weight significantly (p≤0.05) increased in MS containing Ferrioxamine B in comparison with Fe-EDTA. Consequently, Ferrioxamine B is introduced as a cost-effective and applicable Fe source to favour iron deficiency in vitro.
Moazameh Kordjazi, Seyed Hojat Mirsadeghi, Omid Asadi Farsani,
Volume 6, Issue 2 (8-2019)
Abstract
Nowadays, brown macroalgae are considered to be a source of various nutritional and pharmaceutical compounds. In addition, fish roe is very susceptible to spoilage due to its high amount of protein and unsaturated fatty acids. In order to postpone the deterioration of roe-related products, salt and preservatives are utilized. Natural preservatives are recommended because of the adverse effects of chemical additives. Thus, different concentrations of pure alginate (0.1%, 0.2%, 0.3%, 0.4%, 0.5%) were chose and then the 0.2% alginate was selected on the basis of the sensory evaluation of the results. Moreover, the treatments including control (salt-alginate-free), A1 (containing 2.5% salt) A2 (containing 0.2% alginate), A3 (containing 2.5% salt and 0.2% alginate) were used to investigate their preservative effects on fish roe. The quality of rainbow trout roe was assessed within 0, 15, 30, 45 days at refrigerated storage. The treatment A3 showed significant differences in microbial, chemical and sensory results compared with other treatments (p≤0.05). According to the results, salting 2.5% and alginate 0.2% method gave good flavor and increased shelf life to the roe.
Mahnaz Nasre Taheri, Gholamhossein Ebrahimipour, Hossein Sadeghi,
Volume 6, Issue 3 (10-2019)
Abstract
Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic activity of supernatant was measured by caseinolytic method. The effects of pH, temperature, SDS, Tween 80 and EDTA on protease stability and activity were investigated. The detergent compatibility of protease was assayed. On the basis of phylogenetic analysis and morphological as well as biochemical tests, the isolate was identified as a new strain of Brevibacillus borstelensis capable of generating extracellular alkaline protease. The generated protease was determined as alkaline metallo-protease having high activity at 60 oC and pH 9. Moreover, the alkaline protease was stable in the presence of SDS, Tween 80 and H2O2. It is compatible with commercial detergents. Finding proteases capable of degrading proteins in extreme environment (i.e. alkaline pH, high temperature and presence of surfactants) is valuable in biotechnological and industrial practices. Therefore, it can be utilized in detergent formulation in the future.
Parichehr Hanachi, Hojat Sadeghi Ali Abadi, Nasim Ghorbani, Roshanak Zarringhalami, Khadijeh Kiarostami,
Volume 8, Issue 1 (6-2021)
Abstract
From ancient times, plants have been regarded as therapeutic agents, in addition to their usage as food. Plants are rich sources of antioxidant and phenolic compounds. Lavandula angustifolia and Melissa officinalis are medicinal herbs rich in antioxidant compounds. The aim of this study was to compare the antioxidant and anticancer properties of Lavandula angustifolia and Melissa officinalis extracts using aqueous, ethanol and methanol solvents, to select the best extraction methods and solvents and to evaluate the cytotoxic effect of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines. Methanol, aqueous and ethanol extracts were obtained from the dried leaves of the plants and the antioxidant activities of each extract were measured by DPPH and FRAP methods. Finally, the anticancer effects of the extracts on HeLa, OVCAR-3 and MCF-7 cancer cell lines were evaluated by MTT assay in order to identify the most efficient extract. Comparing the results of total antioxidant assay showed that the highest amount belonged to the ethanol extract of Melissa officinalis and Lavandula angustifolia methanol extracts using lyophilization method. The IC50 value of ethanol extract of Melissa officinalis was equal to 0.028 mg/ml on OVCAR-3 cells, which was the best result obtained in comparison with other solvents, and the ethanol extract of Lavandula angustifolia with IC50 = 2.07 mg/ml on MCF-7 cells was the most effective extract among the others. In HeLa cell-line, methanol extract of Lavandula angustifolia with IC50 = 7.36 mg/ml showed the highest cytotoxicity. In this study, for the first time, the effects of different extracts of Lavandula angustifolia and Melissa officinalis on MCF-7, OVCAR-3 and HeLa cancer cells were evaluated and the results showed that ethanol and methanol extracts of these plants had better toxic effect on cancer cells.
