In this study the effect of iron of Fe (So₄)₂ (7H₂O( and probiotics BioPlus 2B on some blood parameters was investigated on 270 of Rutilus frisii kutum fry with total weight of 0.4±0.1 gr and total length of 3.9±0.3 cm  in nine treatments and triplicates during 60 days. Results showed that the highest number of red blood cells (RBCs) (1.65×10⁶± 1.55 per mm³⁾, hemoglobin concentration (7.36±0.15 g/dl), hematocrit concentration (24.03±0.85 %), MCV (1.45±1.48 Fl), MCH (46.29±0.25 pg) and lymphocyte (92±2) belong to treatment 5. The highest level of MCHC (34.65±0.67 g/dl) and the greatest level of white blood cells (6.6±100 per mm³) were observed in treatments 4 and 8, respectively. The findings of this research showed that treatment 5 contained the highest amount of iron (7 mg/kg of feed) and probiotics (CFC 1.6×10⁹) resulted in the highest amount of red blood cells, hemoglobin, hematocrit, MCV, MCH and lymphocytes in this study.