Zahra Noormohammadi, Bahar Ghasemzadeh, Farah Farahani,
Volume 5, Issue 1 (6-2018)
Abstract
Aloe barbadensis is perennial, monocotyledonous, fleshy plant belongs to
Aloaceae family. In this study, somoclonal variations of regenerated
A. barbadensis plants were investigated. The plantlets of forth subculture transferred to the soil for further study. The genomic DNAs of 40 regenerated plantlets were extracted and genetic variations were studied using SPAR markers including RAPD and ISSR primers. The amounts of Aloe gel also were extracted from regenerated
A. vera plants. Average percentage of polymorphism, Shannon index, Nei's genetic diversity and number of effective alleles based on RAPD data were higher than genetic parameters obtained from ISSR data. NJ cluster and STRUCTURE plot based on molecular markers grouped regenerated plants to distinct clusters. AMOVA analysis also showed a significant (P = 0.01) genetic distinction between studied groups. This result also confirmed differentiation of regenerated plants. The amount of Aloe gel in the four groups (based on clustering method) was compared by using analysis of variance (ANOVA). The results showed no significant (P = 0.746) differences between the amount of gel in four group. In total, our findings showed somaclonal variations on genomic level while no significant differences were observed in amount of gel among regenerated Aloe plantlets.
Narjes Rezazadeh Moghadam, Asghar Zamani,
Volume 10, Issue 4 (3-2024)
Abstract
The family Verbenaceae, currently consists of Verbena and Phyla genera in different parts of Iran, especially northern provinces. The frame of this family has undergone significant changes in comparison with the previous references. For example, Vitex and Clerodendrum have been defined as the genera of this family in previous references. Accordingly, in this study, the relationship among these four genera has been evaluated using morphological and leaf anatomical characters of 20 samples. For this purpose, 67 qualitative and quantitative morphological and anatomical traits were used. The analysis of data was performed using R software ver. 4.3.1. For simultaneous analysis of quantitative and qualitative data, Factor Analysis of Mixed Data (FAMD) method was applied. The results of this study indicate the high value of some quantitative traits such as main vein xylem length, seed width, style length and blade width and some qualitative traits such as blade epidermal cells shape, stomata position, two arms of blade position in relation to each other, indumentum type, main vein epidermal cells shape, petal color, blade vascular bundles number, density of indumentum, leaf margin shape and stem branches length in the clustering of the genera. Totally, Analysis of data led to the separation of these genera. In accordance with the phylogenetic studies, Vitex and Clerodendrum show more affinity to each other and are separated from the currently native members of Verbenaceae in Iran, i.e. Verbena and Phyla.
