Showing 3 results for Nepeta
Fatemeh Jafari, Ali Ganjeali, Elham Amjadi,
Volume 10, Issue 4 (12-2023)
Abstract
Nepeta binaludensis jamzad, as a medicinal plant, which is at risk of extinction due to irregular harvesting and damage of its habitat. Four treatments including: 1- inoculation with Azotobacter chooroccum, 2- inoculation with Bacillus cereus and Pseudomonas putida, 3- inoculation with a mix of three bacteria (A. chooroccum + B. cereus + P. putida) and 4 controls (without inoculation), in the form of a completely randomized design with 3 replications in two vegetative growth stages (ten and twenty weeks after sowing). Plant growth-promoting bacteria (PGPB) improved all the evaluated morphological characteristics, including the length and dry weight of the aerial part, total leaf area and root length compared to the control. Also, the results of biochemical investigations showed that in 10 and 20 weeks old plants, the application of PGPB could increase the phenolic and flavonoid compounds and also antioxidant activity. The contain of some elements such as calcium, potassium, phosphorus, iron and magnesium also increased as a result of inoculation with PGPB. Since the PGPB improved the growth and also increased the effective compounds of N. binaludensis plants, they can be introduced as useful bio fertilizers and considered as a good alternative to chemical fertilizers.
Dr. Fatemeh Rabizadeh, Dr. Farzane Bahadori,
Volume 11, Issue 2 (8-2024)
Abstract
Iran, having about 79 species of the genus Lamiaceae, is one of the regions of origin of this genus. About 77% of the identified species of the mentioned genus in Iran have been registered as exclusive species. There is an Iranian Nepeta species, which was previously taken as a separate species in botanical reference sources, but has been reclassified as a variety of Nepeta kotschyi in the flora of Iran (Jamzad, 2012). In this research, the correctness of the taxonomic position of these two species by examining their various morphological and micromorphological traits such as plant height, leaf size, canopy, type of hairs and nuts, using stereo electron microscope (SEM) photos and SPSS statistical studies. The results of morphological and microstructural studies of the two species N. persica and N. kotschyi showed that there is a significant difference in the characteristics such as the shape of the leaf, the hairs of its apertures and also the height of the two species. Although these differences in the size and shape of the seeds were not clearly observed, in this study, both N. kotschyi and N. persica species had reticulated nuts with papilus. Also, the appearance of the long hairs on the leaves of the two species is completely different. The hairs in N. persica species have papilus, but the hairs in N. persica species have a smooth surface. Therefore, it was suggested that the two species are independent.
Golaleh Mostafavi, Asal Tofangchi Mahyari, Seyyedeh Seyyedeh Mahdokht Maddah,
Volume 12, Issue 1 (3-2025)
Abstract
Gastric cancer is one of the most common cancers, causing significant mortality worldwide. Nepeta species, known for their distinct aroma and taste, have garnered attention in the food industry. Given their phenolic compounds, this study aimed to evaluate the cytotoxic effects of Nepeta binaludensis and N. glomerulosa extracts on AGS gastric cancer cell lines and assess changes in BAX gene expression. Both species were collected from the Binalud Mountains in Khorasan, and their hydroalcoholic extracts were prepared via maceration. The amounts of caffeic acid and Rosmarinic acid were measured using HPLC. The extracts' cytotoxic effects were evaluated with the MTT assay, and apoptosis induction was assessed via DAPI staining. Changes in BAX gene expression were measured using real-time PCR. The IC50 values after 48 hours were 3.70 mg/mL for N. binaludensis extract and 6.92 mg/mL for N. glomerulosa extract. Apoptosis induction was observed under both treatments, but N. binaludensis extract, which contained higher Rosmarinic acid, significantly increased BAX expression compared to the control group. Thus, these extracts may inhibit gastric cancer cell growth, with N. binaludensis showing greater efficacy through BAX-mediated apoptosis induction compared to N. glomerulosa.
