Showing 2 results for Clinic
Mahdis Meraji Masouleh Moghaddam, Farhad Mashayekhi, Ziba Zahiri, Akram Eidi,
Volume 8, Issue 4 (12-2021)
Abstract
This study aimed to investigate the polymorphism of matrix metalloproteinase -3 (MMP-3) gene and its expression in the serum of infertile female patients received in vitro fertilization and embryo transfer (IVF-ET). To do so, 100 women with unsuccessful IVF-ET (IVF–) and 100 women with successful IVF-ET procedure and clinical pregnancy (IVF+) were included. Genetic polymorphism and serum concentration of MMP3 were investigated by ARMS-PCR and ELISA, respectively. The results showed no significant association between MMP-3 gene polymorphism and IVF-ET outcome among the two groups studied. However, a significant decrease in the concentration of MMP-3 serum in the IVF– group was observed in comparison with the IVF+ group (P=0.000002). Moreover, we showed that the serum MMP-3 levels in CC, AC and AA genotypes in the IVF– group were 33, 65.33 and 86 ng/ml, respectively. In conclusion, while there is no significant difference between MMP-3 promoter polymorphism and IVF-ET outcome between the IVF+ and IVF- groups, a significant decrease in MMP-3 serum levels in IVF- group was seen as compared with the IVF+ group. It could be also suggested that the CC genotype is associated with a decreased level of MMP-3 serum concentration and may be associated with IVF-ET failure.
Dr Behnaz Bakhshandeh, Alireza Shafizadeh Esfandabadi, Negin Kiaei, Mohammad Hosien Karimi,
Volume 12, Issue 1 (3-2025)
Abstract
Stem cells have received serious attention from the medical industry based on their unique abilities and characteristics for precise and personalized medical approaches. To optimize and modify the behavioral characteristics of these cells for various clinical and research applications, it is usually necessary to create phenotypic changes resulting from permanent or transient genetic manipulations in these cells. Previously radiation and chemical reagents were used for random mutation induction in their genome but today, with the development of targeted modification tools like nucleases, it is possible to change only the desired gene and trait both inside the genome and episomally. Favorable gene transferring is accomplished with the help of different biological, chemical, or physical mediators and procedures. This literature review addressed the importance and necessity of stem cell manipulations, stem cell genetic modification procedures and advantages and disadvantages of each method, as well as available solutions to improve the efficiency and security of mentioned methods for clinical approaches.
