Showing 2 results for Root
Reza Norouzi1, Mesbah Babalar, Masoud Mirmasoumi3 ,
Volume 4, Issue 2 (9-2017)
Abstract
Hairy root induction in plants is the result of the insertion of T-DNA from Agrobacterium rhizogenes into the plant genome. The present study was conducted to investigate the effect of bacterium strain and plant species type on hairy root induction in two endemic (Salvia eremophila and S. reuterana) and five non-endemic (S. macrosiphon, S. multicaulis, S. nemorosa, S. verticellata and S. virigata) Salvia by four bacteria strains including 1724, 2659, ATCC-15834 and A4. Petiole and stem explants were not capable of inducing hairy roots, while almost all leaf segments produced it. Confirmatory studies were carried out by direct detection of inserted rol C by the PCR. The results showed that different Agrobacterium rhizogenes strain and Salvia spices had significant effect on hairy roots number and frequency. The infection of S. macrosiphon via A. rhizogenes strain ATCC15834 showed the highest number of infe-cted roots per explant (5.12 hairy roots) and root frequency (82%). The highest number of hairy root per explant in S. eremophila (3.32 hairy roots) and S. reuterana (3.92 hairy roots) were achieved by inoculation with strain A4. Strain 2659 produced the highest hairy roots number in S. nemorosa (2.6 hairy roots), S. multicaulis (4.36 hairy roots) and S. verticillata (5 hairy roots). Also hairy roots formation occurred at the highest number in S. virigata (3 hairy roots) with infection by strain ATCC15834.
Elaheh Zadeh-Hosseingholi, Nader Chaparzadeh, Samira Mahmudi Aghdam,
Volume 6, Issue 4 (1-2020)
Abstract
Some rhizobacteria have positive effects on plants growth. Syrian bean-caper (Zygophyllum fabago) is a weed plant with medicinal value. This study was conducted to isolate and identify bacteria from Syrian bean-caper rhizosphere. Characteristics associated with plant growth stimulation, such as phosphate and zinc dissolution, production of Indole acetic acid and antifungal activity, were investigated. The isolates were separately inoculated to the plant and after plant root establishment was ensured, their effectiveness in increasing plant growth in greenhouse conditions was measured. Biochemical and molecular identification results showed that five isolates belonged to the genera Bacillus, Pseudomonas, Pantoea, and Brevibacterium. All five isolates showed some degree of plant growth promotion capabilities. Among the isolates, only the genus Bacillus increased the dry weights of plants significantly. The amount of phosphate solubilization for this isolate was 440 μg ml-1 and its acid production in the culture medium was higher than that in other isolates. The isolate had zinc solubilisation capability and produced 3.89 mg ml-1 indole acetic acid. However, this isolate did not show antifungal activity against two fungal pathogens of Aspergillus niger and Botrytis cinerea.
