Volume 6, Issue 2 (8-2019)                   nbr 2019, 6(2): 140-147 | Back to browse issues page

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Baharara J, Ramezani T, Saghiri N, Salek F. Investigation apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells . nbr 2019; 6 (2) :140-147
URL: http://nbr.khu.ac.ir/article-1-2733-en.html
Investigation apoptotic effects of silver nanoparticles coated with Achillea biebersteinii extract on A2780 ovarian cancer cells
J-avad Baharara * , Tayebe Ramezani , Negar Saghiri , Farzane Salek
Mashhad Branch, Islamic Azad University, , baharara78@gmail.com
Abstract:   (4997 Views)

In recent years using of silver nanoparticles due to its unique properties was increased. Medical plant, the Achillea biebersteinii is rich in anti-cancers compounds. The aim of this study was to investigate the cytotoxicity effects of AgNPs synthesized using Achillea biebersteinii extract on human ovarian cancer A2780 cells. Cytotoxic effects of AgNPs with MTT test was performed at 48 hours in concentration of 2, 4, 6, 8, 16 and 32 µg/ml. To study the cell death induced by AgNPs DAPI, acridine orange (AO)/ Propidium iodide (PI) staining and Annexin v/ Propidium iodide assay and activation of caspase 3 and 9 were assessed. Results: The results showed that the synthesized AgNPs decreased cell viability dose dependently, calculated inhibitory concentration of 50 % (IC50) was 4 µg/ml.  The results from AO / PI, DAPI staining, Annexin V / PI showed that in treated cells the percentage of apoptotic cells compared with control increased. In addition, the treated cells showed increased activation of caspase 3/9. Hence silver nanoparticles induced cell death through the caspase dependent pathway. The results suggest that AgNPs synthesized using Achillea biebersteinii   extract exert their cytotoxic effect by inducing apoptosis.

Keywords: Nanoparticles, Green Synthesis, Medical plant
Full-Text [PDF 954 kb]   (1761 Downloads)    
Type of Study: Original Article | Subject: Cell and Molecular Biology
Received: 2017/01/18 | Revised: 2019/09/24 | Accepted: 2018/04/17 | Published: 2019/07/30 | ePublished: 2019/07/30
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