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Khodavandi P, Alizadeh F, Khodavandi A. The multidrug resistance and production of extended-spectrum beta-lactamases ctx-m, per and ver in Escherichia coli isolates derived from raw dairy samples. nbr 2020; 7 (1) :46-54
URL: http://nbr.khu.ac.ir/article-1-2931-en.html
The multidrug resistance and production of extended-spectrum beta-lactamases ctx-m, per and ver in Escherichia coli isolates derived from raw dairy samples
Pouria Khodavandi , Fahimeh Alizadeh , Alireza Khodavandi *
Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran , alireza_khodavandi@yahoo.com
Abstract:   (4256 Views)
The occurrence of extended-spectrum beta-lactamases-producing bacteria is an important public health issue. The aim of this study was to investigate phenotypic and genotypic characteristics regarding the presence of extended spectrum β-lactamase ctx-m, per and ver producing Escherichia coli isolated from raw dairy samples. For this purpose, E. coli were isolated from 247 raw dairy samples (milk and cheese) in Yasooj in 2015-2017, and the isolates were screened for antibiotic resistance, extended spectrum β-lactamase and the presence of ctx-m, per and ver. In total, 200 isolates were selected. The highest frequency of resistance in isolates was against tetracycline (96.5%) and ampicillin (95.5%) antibiotics and the lowest against imipenem (12.5%), In addition, multidrug resistance against four or more antibiotics was observed in some isolates. Extended spectrum β-lactamase resistance was detected in 86 isolates (43%) and ctx-m, per and ver genes were detected in 82, 0 and 7 E. coli isolates, respectively. These findings demonstrated that raw dairy products may be reservoirs for the dissemination of β-lactam antibiotics and that resistance genes could be transmitted to humans through the food chain.

 
Keywords: antibiotic, bacteria, gene, raw milk, unprocessed cheese
Full-Text [PDF 871 kb]   (1338 Downloads)    
Type of Study: Original Article | Subject: Microbiology
Received: 2017/08/12 | Revised: 2021/06/1 | Accepted: 2019/11/4 | Published: 2020/03/31 | ePublished: 2020/03/31
References
1. Bonnet, R. 2004. Growing group of extended-spectrum β-lactamases: the CTX-M enzymes. Antimicrob. Agents Chemother. 48: 1-14. [DOI:10.1128/AAC.48.1.1-14.2004]
2. Bonyadian, M., Ebrahimi, A. & Jamali, M. 2013. Study on the antibiotic resistance of E. coli isolated from raw milk and unpasteurized cheese and survey on resistance transmission to E. coli O2: K12. Iranian J. Vet. Sci. 7: 26-31.
3. Braun, S.D., Ahmed, M.F., El-Adawy, H., Hotzel, H., Engelmann, I., Weiß, D., Monecke, S. & Ehricht, R. 2016. Surveillance of extended-spectrum beta-lactamase-producing Escherichia coli in dairy cattle farms in the Nile Delta, Egypt. Front Microbiol. 7: 1-14. [DOI:10.3389/fmicb.2016.01020]
4. Clinical Laboratory Standards Institute (CLSI). 2014. Performance standards for antimicrobial susceptibility testing; Supplement M100-S24.
5. Doi, Y., Paterson, D.L., Egea, P., Pascual, A., Lopez-Cerero, L., Navarro, M.D., Adams-Haduch, J.M., Qureshi, Z.A., Sidjabat, H.E. & Rodriguez-Bano, J. 2010. Extended-spectrum and CMY-type beta-lactamase-producing Escherichia coli in clinical samples and retail meat from Pittsburgh, USA and Seville, Spain. Clin. Microbiol. Infect. 16: 33-38. [DOI:10.1111/j.1469-0691.2009.03001.x]
6. Edelstein, M., Pimkin, M., Palagin, I., Edelstein, I. & Stratchounski, L. 2003. Prevalence and molecular epidemiology of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella pneumoniae in Russian hospitals. Antimicrob. Agents Chemother. 47: 3724-3732. [DOI:10.1128/AAC.47.12.3724-3732.2003]
7. Geser, N., Stephan, R. & Hächler, H. 2012. Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Vet. Res. 8: 21. [DOI:10.1186/1746-6148-8-21]
8. Holoda, E., Vu-Khac, H., Andraskova, S., Chomova, Z., Wantrubova, A., Krajnak, M. & Pilipcinec, E. 2005. PCR assay for detection and differentiation of K88ab(1), K88ab(2), K88ac, and K88ad fimbrial adhesins in E. coli strains isolated from diarrheic piglets. Folia Microbiol (Praha) 50: 107-112. [DOI:10.1007/BF02931457]
9. Jiang, X., Zhang, Z., Li, M., Zhou, D., Ruan, F. & Lu, Y. 2006. Detection of extended-spectrum beta-lactamases in clinical isolates of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 50: 2990-2995. [DOI:10.1128/AAC.01511-05]
10. Kim, S., Hu, J., Gautom, R., Kim, J., Lee, B. & Boyle, D.S. 2007. CTX-M extended-spectrum β-lactamases, Washington State. Emerg. Infect. Dis. 13: 513-516. [DOI:10.3201/eid1303.060479]
11. Kurekci, C., Arkadaş, M. & Avsar, Y.K. 2016. Occurrence, genetic characterization and antimicrobial resistance of extended spectrum β-lactamase producing Escherichia coli isolated from Sürk samples, a traditional Turkish cheese. J. Food Meas. Charact. 10: 709-714. [DOI:10.1007/s11694-016-9355-7]
12. Raveh, D., Yinnon, A.M., Broide, E. & Rudensky, B. 2007. Susceptibilities of ESBL-producing Enterobac- teriaceae to ertapenem, meropenem and piperacillin-tazobactam with and without clavulanic acid. Chemotherapy 53: 185-189. [DOI:10.1159/000100516]
13. Rawat, D. & Nair, D. 2010. Extended-spectrum β-lactamases in gram negative bacteria. J. Glob Infect. Dis. 2: 263-274. [DOI:10.4103/0974-777X.68531]
14. Rupp, M.E. & Fey, P.D. 2003. Extended spectrum betalactamase (ESBL)-producing Enterobacteriaceae: considerations for diagnosis, prevention and drug treatment. Drugs 63: 353-365. [DOI:10.2165/00003495-200363040-00002]
15. Schmid, A., Hormansdorfer, S., Messelhäusser, U., Kasbohrer, A., Sauter-Louis, C. & Mansfeld, R. 2013. Prevalence of extended-spectrum β-lactamase-producing Escherichia coli on Bavarian dairy and beef cattle farms. Appl. Environ. Microbiol. 79: 3027-3032. [DOI:10.1128/AEM.00204-13]
16. Shaikh, S., Fatima, J., Shakil, S., Rizvi, S.M. & Kamal, MA. 2015. Antibiotic resistance and extended spectrum beta-lactamases: types, epidemiology and treatment. Saudi J. Biol. Sci. 22: 90-101. [DOI:10.1016/j.sjbs.2014.08.002]
17. Skockova, A., Bogdanovicova, K., Kolackova, I., Karpískova, R. 2015. Antimicrobial-resistant and extended-spectrum β-lactamase-producing Escherichia coli in raw cow's milk. J. Food Protect. 78: 72-77. [DOI:10.4315/0362-028X.JFP-14-250]
18. Snow, L.C., Warner, R.G., Cheney, T., Wearing, H., Stokes, M., Harris, K., Teale, C. J. & Coldham, N.G. 2012. Risk factors associated with extended spectrum beta-lactamase Escherichia coli (CTX-M) on dairy farms in North West England and North Wales. Prev. Vet. Med. 106: 225-234. [DOI:10.1016/j.prevetmed.2012.03.009]
19. Suardana, I.W. 2014. Analysis of nucleotide sequences of the 16S rRNA gene of novel Escherichia coli strains isolated from feces of human and bali cattle. J. Nucleic Acids 2014: 1-7. [DOI:10.1155/2014/475754]
20. Vrabec, M., Lovayova, V., Dudrikova, K., Gallo, J. & Dudrikova, E. 2015. Antibiotic resistance and prevalence of Enterococcus spp. and Escherichia coli isolated from Bryndza cheese. Italian J. Anim. Sci. 14: 609-614. [DOI:10.4081/ijas.2015.3968]
21. Zamani, K., Emami, A., Bazargani, A. & Moattari, A. 2015. Phenotypic and molecular characterization of CTX-M extended-spectrum beta-lactamase-producing Escherichia coli isolates in Shiraz, Iran. Rev. Soc. Bras. Med. Trop. 48: 479-482. [DOI:10.1590/0037-8682-0315-2014]
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