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Darvishi F. The production of Yarrowia lipolytica lipase powder by improved spray-drying method. nbr 2019; 6 (2) :261-267
URL: http://nbr.khu.ac.ir/article-1-3075-en.html
The production of Yarrowia lipolytica lipase powder by improved spray-drying method
Farshad Darvishi *
University of Maragheh , f.darvishi@ymail.com
Abstract:   (4650 Views)

Lipase is used in the production of foods, flavor enhancers, detergents, cosmetics and pharmaceuticals. A common impediment to the production of commercial enzymes is their low-stability aqueous solutions. In this study, the downstream process was investigated to obtain a stable spray-dried lipase powder of Yarrowia lipolytica. The enzyme solution samples were supplemented with different concentrations of Arabic gum and milk powder to spray-drying. Samples were dried in a pilot spray dryer at inlet and outlet temperatures of 175 and 85 °C, respectively, at a flow rate of 15 liters per hour. The best lipase powder obtained from spray-drying with 3% of Arabic gum and 9% of milk powder formulation as compared with other formulations. Results showed that spray-dried lipase powders of Y. lipolytica had a good yield suitable for various applications.
 
 

Keywords: yeast, enzyme, production, formulation, downstream process
Full-Text [PDF 711 kb]   (1354 Downloads)    
Type of Study: Original Article | Subject: Microbiology
Received: 2018/01/26 | Revised: 2019/08/27 | Accepted: 2019/01/27 | Published: 2019/07/30 | ePublished: 2019/07/30
References
1. Alloue, W.A.M., Destain, J., Amighi, K. and Thonart, P. 2007. Storage of Yarrowia lipolytica lipase after spray-drying in the presence of additives. - Process Biochem. 42: 1357-1361. [DOI:10.1016/j.procbio.2007.05.024]
2. Amaral, P.F.F., de Almeida, A.P.R., Peixoto, T., Rocha-Leao, M.H.M., Coutinho, J.A.P. and Coelho, M.A.Z. 2007. Beneficial effects of enhanced aeration using perfluorodecalin in Yarrowia lipolytica cultures for lipase production. - World J. Microbiol. Biotechnol. 23: 339-344. [DOI:10.1007/s11274-006-9229-y]
3. Bare, G., Diakiese, A., Zgoulli, S., Sarri, A., Gerday, C. and Thonart, P. 1999. Modification of the thermo resistance to spray-drying of a cold-adapted subtilisin by genetic engineering. - Appl. Biochem. Biotechnol. 79: 857-865. [DOI:10.1007/978-1-4612-1604-9_78]
4. Darvishi, F., Fathi, Z., Ariana, M. and Moradi, H. 2017. Yarrowia lipolytica as a workhorse for biofuel production. - Biochem. Engin. J. 127: 87-96. [DOI:10.1016/j.bej.2017.08.013]
5. Darvishi, F. 2012. Expression of native and mutant extracellular lipases from Yarrowia lipolytica in Saccharomyces cerevisiae. - Microbial Biotechnol. 5: 634-641. [DOI:10.1111/j.1751-7915.2012.00354.x]
6. Darvishi, F., Destain, J., Nahvi, I., Thonart, P. and Zarkesh-Esfahani, H. 2012. Effect of additives on freeze-drying and storage of Yarrowia lipolytica lipase. - Appl. Biochem. Biotechnol. 168: 1101-1107. [DOI:10.1007/s12010-012-9844-z]
7. Darvishi, F., Destain, J., Nahvi, I., Thonart, P. and Zarkesh-Esfahani, H. 2011. High-level production of extracellular lipase by Yarrowia lipolytica mutants from methyl oleate. - New Biotechnol. 28: 756-760. [DOI:10.1016/j.nbt.2011.02.002]
8. Destain, J., Roblain, D. and Thonart, P. 1997. Improvement of lipase production from Yarrowia lipolytica. - Biotechnol. Lett. 19: 105-107. [DOI:10.1023/A:1018339709368]
9. Dokic, P., Jakovljevic, J. and Dokic-Baucal, L.J. 1998. Molecular characteristics of maltodextrins and rheological behaviour of diluted and concentrated solutions. - Colloids Surf. A. 141: 435-440. [DOI:10.1016/S0927-7757(97)00118-0]
10. Fickers, P., Benetti, P.H., Wache, Y., Marty, A., Mauersberger, S., Smit, M.S. and Nicaud, J.M. 2005. Hydrophobic substrate utilisation by the yeast Yarrowia lipolytica, and its potential applications. - FEMS Yeast Res. 5: 527-543. [DOI:10.1016/j.femsyr.2004.09.004]
11. Mafakher, L., Mirbagheri, M., Darvishi, F., Nahvi, I., Zarkesh-Esfahani, H. and Emtiazi, G. 2010. Isolation of lipase and citric acid producing yeasts from agro-industrial wastewater.- New Biotechnol. 27: 337-340. [DOI:10.1016/j.nbt.2010.04.006]
12. Millqvist-Fureby, A., Malmsten, M. and Bergenstahl, B. 1999. Spray-drying of trypsin surface characterization and activity preservation. - Int. J. Pharm. 188: 243-253. [DOI:10.1016/S0378-5173(99)00226-4]
13. Mirbagheri, M., Nahvi, I., Emtiazi, G., Mafakher, L. and Darvishi, F. 2012. Taxonomic characterization and potential biotechnological applications of Yarrowia lipolytica isolated from meat and meat products.- Jundishapur J. Microbiol. 5: 346-351.
14. Shiga, H., Yoshii, H. and Nishiyama, T. 2001. Flavor encapsulation and release characteristics of spray-dried powder by the blended encapsulant of cyclodextrin and gum arabic. - Drying Technol. 19: 1385-1395. [DOI:10.1081/DRT-100105295]
15. Werner, L., Latzko, F. and Hampel, W. 1993. Spray-drying of yeast-lytic enzymes from Arthrobacter sp. -Biotechnol. Techniq. 7: 663-666. [DOI:10.1007/BF00151866]
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